Nice run. I’m not particularly familiar with this game but one thing I can say is that you can fully skip dialogue boxes with the start button. That’s the only improvement I can think of however, the rest of the run seems clean.
Have you also read the forum thread? FriiedPorkchop discovered some glitches involving tobogganing mode that help preserve speed and can even clip through things, skipping part of Arctic Pen 3. The Giraffe Pen demo encode used the mGBA core, while the BK2 from the later post used VBA-Next in BizHawk 1.11.1, and I couldn't get it to sync in BizHawk 2.3.2 so it might require an older version. I did format and paste its input into a GBMV but only the very first level (Mr. Chew) syncs, the rest may need to be resynced for every room if you want to directly compare strategies.
In Zebra Pen 1, did you skip a dance at the end? It sounds like Private's voice starts to play, then cuts out and the BGM restarts in the second room. Something similar happens at the end of Monkey Cages 2 with Alex. At 1:27, you toboggan across part of that pond, but on revisits at 10:37 and 16:37 you don't. Could it also be faster to toboggan across at 13:50, 17:20, etc? Otherwise, looks good and it's great you shaved off almost a full minute.
