Got around to watching this run. Here's my review of this TAS:
There are a couple of minor things that bother me though they probably only save frames rather than seconds and minutes:
- When turning around on the ground, Samus can shoot to reduce the frames of the turnaround animation which saves a very minor bit of frames. This is most noticeable after picking up some of the items in the run.
- There were a few instances where the morph ball bounces when landing from a great height that seem preventable here and there but I'm not completely certain there's a way to prevent all of those.
Otherwise the optimization seems pretty solid overall from my understanding of the GBA Metroid games. I can't say for certain if the route is completely optimal but being over 6 minutes faster IGT than the
100% world record as of this post seems like it's pretty solid as well. It would help if the author did expand their submission notes to have info about the routing choices, as it would definitely help out with understanding the run.
EDIT: For this run to sync, this requires the version 1.1 patch of the hack. There's a checksum mismatch and desync shortly after the first boss when replaying this movie with the version 1.0 patch.