Well, it is about time to post a
WIP of my progress. For those who don't know, Baxter managed to convince me to do a run of this game :p
I am at the beginning of the water stage and am 375 frames ahead of the current published run.
A new, bizarre glitch was used to save time on boss fights. While in the air, pressing up+down+right+b for two frames, you glitch the sprite data of your character and create a "floating weapon" on the screen that can damage enemies. For don, this is a sprite of leo's sword. The weapon will last until you press B again.
Also, I took damage early on in order to get the damage boost to don's attacks. When your energy is under %50, your attacks are more powerful. This allowed me to kill all of the bosses so far in less hits, which saves 48 frames per hit saved.
There are lot's of tricks posted by spider-waffle that should saved even more time.
Please feel free to leave comments & suggestions.