While I will say I did enjoy the ways you chose to entertain your audience with the jump mechanics, and your abuse of Dixie's slap boost mechanic, this run is still highly suboptimal for many reasons. You did improve on some things, like using the US version and doing the tricks you see in Avanor's speedrun, such as exiting course at the flagpoles, but there are still some things that need work.
But there are still some things that stand out to me immediately about the run as suboptimal. I'm only going to go over the first world, as it pretty much sums up how the rest of this run should be treated. Here are some obvious problems with this run:
* At least 10 frames that feos saved on menuing. Stop turboing stuff.
* Though it could be seen as a speed/entertainment tradeoff, I don't believe in those, so it's a timewaster to me. Typing in a name there wastes a lot of time.
* Frame 1745: He just went toward that curve, which wastes quite a lot of frames. He didn't go around it.
* Frame 2180: He does a slap with Dixie at the beginning of the level. Every time you slap with Dixie (with the exception of hitting enemies), it is WAY SLOWER. What you're supposed to do instead to fix this problem is hold B before entering the level, rather than on the frames before it starts.
* Frame 3200: That's an immediately noticeable problem right there. Spectrum doesn't get the 5 silver coins up above, on the right side of that shelter. There is a cannon that you do a teamjump to get up to that blasts you through that entire portion AND gives you 5 silver coins. It's very, very clearly faster, and it's unquestionable in my mind.
* Beginning of the mill level: You used Dixie's slap again, but you just jumped this time, which still wastes some frame amount.
* I need to mention that every time you use Dixie's helicopter, it wastes 3 frames, because that's the amount of wait time you have to use to do it. Don't use Dixie's helicopter unless it is REALLY necessary.
* Frame 8038: I know for fact you could find a faster coin than this one to grab. And why is it the first coin you ever grab?
* Frame 11218:
https://www.youtube.com/watch?v=_tbo5i-0nHg See this video for a faster method.
* And you keep doing Dixie's slap at the beginning of the minigames too...
* 17100: At the last spike thingy, you should hit it at the very edge of your shield, saving the most time, I believe.
* 19400: You did the thing again where you went against the curves and wasted time.
* Beginning of Level 3: Okay, you switch to Kiddie, and don't use Kiddie's rolling mechanic constantly, which is optimal. Kiddie's rolls save more time than when he just runs regularly.
* 23607: That boost was good, but you should've waited a lot longer before cancelling it.
* 23900: You missed that coin that was literally right there.
* 26225: You didn't jump soon enough, so there were some frames where you were still on that slope, which wastes time.
* 30600: You were on the slope right there again for too long.
* 31970: It may be faster in the SNES version, but not in GBA I believe. Doing this level twice is far slower in this version AFAIK.
* When you entered reentered the level, you jumped while on that elevator in an attempt to entertain. That's a HUGE no-no. See, every time you jump, the elevator slows down just a little, which delays the amount of time it takes you to get up where you need to go.
* 34700: You jumped too late.
* I didn't mention all the specific times you still used Dixie's slap at the beginning of every level, but you did do that.
Things you need to work on are your routing for the silver coins. The silver coin routing is very complex, as there are so many of them in the game but only 75 needed. Really, some of those basic improvements I just mentioned, like avoiding the curves being faster when swimming in those parts I mentioned, and not slapping with Dixie's hair at the beginning of levels, are things that a TASer of the game should just know.
TASing is not something you can just complete in a week, or even two weeks, like TASing is, ESPECIALLY in the case of this very complex game. TASing is very difficult, time-consuming, and HAS to be perfect. I assume you are used to the non-TAS speedrunning setting, due to the resemblance of this TAS to a speedrun of the game and because of the way you're TASing it in general. Sure, you can learn a speedrun in a short amount of time, and don't have to spend nearly as much time mending your mistakes, etc., but TASing is a LOT more than that. You have to know your game so well that there couldn't even be a doubt in your mind that it was even a couple frames short of perfect. The TASing setting is very different from the speedrunning setting, and it probably takes some time to get used to. But still, work on getting used to it.
The silver coin routing, I must stress, is a HUGE deal, and you CAN'T do that in two weeks. I actually literally have to go through each silver coin in the game that even could be used in the run, and test it out and record how many frames it wastes. Basically, I have to find the 75 coins in the game that waste the least amount of frames. It's pretty clear that you didn't go through that process yet, and it's also clear that there are some coins you just flat-out missed. And this is all stuff you have to do OUTSIDE of TASing itself; you actually have to do this in some sort of testrun or just without recording at all.
I advise that you please do not submit more runs of this game here. Instead, please use the TASVideos user files. That way, someone (like me) can check over your work and tell you what went wrong, so that you don't keep getting your runs rejected, and so that I don't have to go back and look for suboptimality anymore times to prove to the judges that it should be rejected.
Not trying to be mean here, but please, do me that favor. Do us all that favor.
Since I'm also TASing this game, you might want to consider joining forces with me on this project, since you clearly have a lot of knowledge about the game's mechanics and are interested in TASing it. PM me or add me on Skype (below) for details. I can also give you my Discord from there. If you'd like feedback on the entire TAS, rather than just that one world, tell me and I'll take the time to do a complete analysis.
And really, this kind of TAS would be best if it were a coop submission.